Project

Part:BBa_I13537:Design

Designed by: Josh Michener   Group: iGEM04_MIT   (2005-08-22)


Two promoter screening plasmid v1.0


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1247
    Illegal NheI site found at 2373
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1367
    Illegal BamHI site found at 1187
    Illegal BamHI site found at 2313
    Illegal XhoI site found at 1345
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1952
    Illegal AgeI site found at 2064
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3048
    Illegal SapI site found at 961


Design Notes

Source

References